Epigenetic Manipulation Facilitates the Generation of Skeletal Muscle Cells from Pluripotent Stem Cells

Figure 4

Efficient myogenic differentiation of hESCs by synRNA-mediated overexpression of JMJD3c and MYOD1. Transfection of synRNAs encoding JMJD3c and MYOD1 directly converts hESCs into MHC-positive myogenic cells for 5 days post differentiation. The differentiation efficiency was much higher in the JMJD3c/MYOD1-overexpressing hESCs compared with the MYOD1-overexpressing hESCs. MHC, green. DAPI, blue.