Induced Pluripotent Stem Cells Reduce Progression of Experimental Chronic Kidney Disease but Develop Wilms’ Tumors

Figure 4

(a) Tumor cells infiltrating the renal interstitium with PCNA-positive nuclei. Notice that the majority of tumor cells were stained in brown by the PCNA antibody. Immunoperoxidase reaction using PCNA antibody revealed with the chromogen 3,3′-diaminobenzidine (DAB) in brown, followed by periodic acid-Schiff (PAS) staining. Bar: 100 μm. (b) Tumor cells arranged as either isolated or grouped cells surrounded by a dense PAS reactive extracellular matrix, some with nuclei positive for PCNA antibody. PCNA immunoperoxidase reaction revealed with DAB and followed by PAS staining. Bar: 100 μm. (c) In an area of the tumor, the presence of a nodular structure composed of PAS-positive ECM. PCNA immunohistochemistry revealed with DAB and followed by PAS staining. Bar: 100 μm. (d) Tumor area composed by a dense PAS+ ECM, with tumor cells immersed resembling cartilaginous tissue. PCNA immunohistochemistry revealed with DAB and followed by PAS staining. Bar: 50 μm.

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