Research Article
Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia
Figure 11
Immunohistochemistry (IHC) and staining with hematoxylin and eosin (HE) from the histological sections of the iPSC PBscd08cl02HU teratoma, demonstrating the staining for the 3 germ layers. (a) (I) Image identifying muscle tissue with elongated cells like muscle fibers, of mesodermal origin, and respiratory and guandular epithelial tissue, both of endodermal origin. (II, III) Images demonstrating the positive staining for α-SMA, confirming the mesodermal origin. (IV) Image of HE staining. (V, VI, and VII) Images demonstrating the positive staining for AFP, confirming that they are tissues from the endodermal lineage. (b) (I) Images of HE staining, evidencing the neural tissue region. (II, III) Images of IHQ showing positive staining for Nestin, confirming the ectodermal origin of the tissue found.
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