Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Figure 13

Analyses performed by the hPSC Scorecard analysis software from data of the TaqMan hPSC Scorecard test with the iPSC PBscd01 and the iPSC EB PBscd01. (a) First box: analysis of the gene expression of self-renewal and differentiation markers in EB of iPSC PBscd01, confirming its differentiated cell characteristic, with positivity for markers of the 3 germ layers, highlighting mesoderm. Second box: analysis of the gene expression of self-renewal and differentiation markers in the iPSC PBscd01, confirming its pluripotent characteristic, being negative for markers of the three germ layers, that is, differentiation markers. (b) Gene expression analysis of several markers of self-renewal and differentiation, the latter represented by gene characteristic of the 3 germ layers, ectoderm, mesoderm, and endoderm. The upper line details the EB iPSC PBscd01 experiment and demonstrates the upregulation (red, light red, and light pink) of the genes of the 3 germ layers, mainly the mesodermal, and the downregulation (dark blue, blue, and light blue) of self-renewing and pluripotency genes, in the first column. The bottom line details the iPSC PBscd01 experiment and demonstrates the downregulation of genes from the 3 germ layers, especially the endoderm, and upregulation of self-renewal and pluripotency genes.

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