Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Figure 16

(a) Image of 1.5% agarose gel stained with ethidium bromide representative of the electrophoresis of the pEB-Tg vector amplification reaction in the iPSC lines generated PBscd. M, 100 bp ladder; 1, positive control MNC PBscd; 2, sample iPSC PBscd02 P6; 3, sample iPSC PBscd01 P5; 4, sample iPSC PBscd03 P4; 5, sample iPSC PBscd03 P8; 6, sample iPSC PBscd03 P14; 7, negative reaction control. (b) Image of 1.5% agarose gel stained with ethidium bromide representative of the electrophoresis of the pEB-Tg vector amplification reaction in the iPSC line PBscd01 to confirm vector deletion. pb, number of base pairs; M, 100 pb ladder; 1, positive control MNC PBscd; 2, iPSC sample PBscd01 P5; 3, sample iPSC PBscd01 P10; 4, negative reaction control.

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