Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Figure 18

Differentiation process. (a) Schematic image representing the whole process of hematopoietic differentiation until obtaining hematopoietic progenitors, passing through a phase of adaptation of the iPSC prior to differentiation. (b) Predifferentiation process, leading the cells to adapt to the enzymatic picking. (I, II) Cells in the process of adaptation, showing some colonies, but many individualized and differentiated cells. (III, IV) Cells adapted to the enzymatic picking, showing that the colonies acquire more rounded morphology, and few or no individual cells are visible between the colonies. Images in phase contrast. Optical microscopy.

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