Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Figure 20

Immunophenotyping of cells obtained after induction of differentiation, by flow cytometry, showing the markers evaluated and the percentage of positive cells for each marker. On day 0, immunophenotyping of pluripotent cells prior to induction of differentiation showed the high percentage of pluripotency markers, OCT4, SOX2, and NANOG. After 4 days of induction of differentiation, immunophenotyping of the cells showed decreasing pluripotency markers. Analyses are carried out in FACSCalibur.