Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Figure 22

Immunophenotyping of cells obtained after induction of differentiation, by flow cytometry, showing the markers evaluated and the percentage of positive cells for each marker. (a) Immunophenotyping of the cells obtained after 11 days of induction of differentiation, showing the drop in the percentage of cells positive for markers of hematopoietic progenitors, when compared with D + 8. (b) Immunophenotyping of the cells obtained after 14 days of induction of differentiation and end of the experiment, showing loss of almost all markers, remaining only 30.3% of the CD34-positive cells when analyzed separately and 24.4% of the supernatant cells. Cells were positive for CD34 when analyzed separately. Cells were analyzed in FACSAria and analyses were performed in FlowJo software.

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