Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Figure 4

Reprogramming process of iPSC PBscd02, showing the alteration in the cell morphology, passing from individualized cells with growth in suspension to cells with growth in colonies. (a) PBMCscd02 with 6 days of expansion in specific medium for lymphoid cell depletion and nonlymphoid cell enrichment. (b) Emergence of the first colonies on MEF, 11 days after the episomal transfection. (c) Colony growth on MEF, with 13 days postnucleoporation culture, before the first peal. (d) Colony established after the first peal for matrix Matrigel. Images in phase contrast. Optical microscopy.

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