Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Figure 9

In vitro differentiation assay. (a) Confirmation of the differentiation potential of iPSC PBscd01 through spontaneous differentiation and formation of EB. Images show the in vitro morphological changes of iPSC PBscd01. (I, II) EB formed by suspension culture. (III, IV) EB after spontaneous differentiation in adherence. Phase contrast images. Optical microscopy. (b) Differentiation analyzed for the presentation of the markers of cells of the 3 germ layers, by qPCR of the EB. Individual qPCR reactions were normalized against internal controls (GAPDH and β-actin) and plotted against the level of parental cell expression (MNC PBscd01).

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