Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia

Table 1

Set of primers used in the screening of spontaneous integration of the vectors used to reprogram iPSCs.

Primer	Sequence	Vector	μg DNA	Ta (°C)
EBNA_1 forward	5′-TTTAATACGATTGAGGGCGTCT-3′	pEB-C5	0,1	57
EBNA_1 reverse	5′-GGTTTTGAAGGATGCGATTAAG-3′	pEB-C5
Tg foward	5′-GCCAGGTGGGTTAAAGGAGC-3′	pEB-Tg	0,02	60
Tg reverse	5′-GGTACTTATAGTGGCTGGGCTGT-3′	pEB-Tg

Ta: annealing temperature.