Research Article
Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia
Table 1
Set of primers used in the screening of spontaneous integration of the vectors used to reprogram iPSCs.
| Primer | Sequence | Vector | μg DNA | Ta (°C) |
| EBNA_1 forward | 5′-TTTAATACGATTGAGGGCGTCT-3′ | pEB-C5 | 0,1 | 57 | EBNA_1 reverse | 5′-GGTTTTGAAGGATGCGATTAAG-3′ | pEB-C5 | Tg foward | 5′-GCCAGGTGGGTTAAAGGAGC-3′ | pEB-Tg | 0,02 | 60 | Tg reverse | 5′-GGTACTTATAGTGGCTGGGCTGT-3′ | pEB-Tg |
|
|
Ta: annealing temperature.
|