Research Article
Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia
Table 2
Efficiency of reprogramming of MNCs by episomal vectors, showing the details of the 6 experiments.
| | iPSC line | Cell source | Treatment with HU | Age | Number of transfected cells | Days of expansion | Number of colonies | First picking | Reprogramming efficiency (%) |
| | PBscd01 | HbSS (F) | No | 20 | 2,5 × 106 | 14 | 24 | Day 14 | 0.0075 | | PBscd02 | HbSS (F) | No | 28 | 2,5 × 106 | 14 | 12 | Day 15 | 0.0046 | | PBscd03 | HbSS (M) | No | 30 | 2,0 × 106 | 12 | 5 | Day 15 | 0.0042 | | PBscd06HU | HbSS (M) | Yes | 32 | 2,5 × 106 | 14 | No colonies | — | 0 | | PBscd07HU | HbSS (M) | Yes | 28 | 2,4 × 106 | 14 | No colonies | — | 0 | | PBscd08cl02HU | HbSS (M) | Yes | 19 | 1,9 × 106 | 14 | 4 | Day 18 | 0.0011 | | PBscd08cl03HU | HbSS (M) | Yes | 19 | 1,9 × 106 | 14 | 4 | Day 18 | 0.0011 | | PBscd08cl04HU | HbSS (M) | Yes | 19 | 1,9 × 106 | 14 | 4 | Day 18 | 0.0011 |
|
|
PB: peripheral blood; scd: sickle cell disease; Hb: hemoglobin; S: mutated hemoglobin; F: female; M: male; HU: hydroxyurea.
|
