Research Article
Induced Pluripotent Stem Cell for the Study and Treatment of Sickle Cell Anemia
Table 4
Summary of percentages of positive cells for each marker on specific days of differentiation.
| | Markers | D + 0 | D + 4 | D + 8 | D + 11 | D + 14 EB | D + 14 supernatant |
| | OCT4 | 86.86% | 6.8% | — | — | — | — | | SOX2 | 98.34% | 46% | — | — | — | — | | NANOG | 87.4% | 2% | — | — | — | — | | CD31 | — | 0.6% | 22.1% | 0% | — | — | | CD144 | — | 0.1% | 5.7% | 0% | — | — | | CD45 | — | 0% | 77.2% | 0.6% | 0.6% | 0.8% | | CD34 APC | — | 3.4% | 58.3% | 27% | 24% | 30.3% | | CD135 | — | 0% | 6.3% | 0% | 0% | 0% | | CD43 | — | 3.4% | 29% | 10% | 0% | 0% | | KDR | — | 1% | 1.8% | 0% | — | — | | CD235a | — | 19% | 63.3% | 5% | 0% | 0% | | CD38 | — | — | 52.5% | 0% | 0% | 0% | | CD117 | —— | — | 84% | 0% | 0% | 0% | | CD41a | — | — | — | — | 0% | 0% | | CD42a | — | — | — | — | 0% | 0% |
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EB: embryoid body; APC: allophycocyanin; KDR: kinase insert domain receptor.
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