Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

Figure 1

Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and HTB82 cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.

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