Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

Figure 2

Self-renewal capacity of cells contained in RMS holoclones and spheres. ((a) and (b)) Initial percentage of the three types of clones generated in CW9019 and HTB82 cell lines, respectively. Representative images and quantification of secondary clones formed from holoclones (c and d), meroclones (e and f), and paraclones (g and h) in CW9019 and HTB82 cell lines, respectively. Enrichment in the secondary sphere fraction obtained in CW9019 (i) and HTB82 (j) cell lines. All experiments were tested in triplicate. Significance: ; .

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