Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

Figure 6

Holoclones showed induction of stem cell markers. mRNA levels of OCT4, NANOG, PAX7, and KITLG were evaluated in CW9019 and HTB82 cell lines. The expression levels of these markers were evaluated by conventional RT-PCR in total cell lines (line) and holoclones of first (holo1) and second (holo2) passages.