NURR1 Downregulation Favors Osteoblastic Differentiation of MSCs

Figure 4

Effect of NURR1 downregulation on ALP and mineralization. (a) ALP histochemical assay (purple staining) performed on DPSCs transfected with NURR1-specific siRNA or scrambled sequences and maintained in osteogenic conditions for 7 days. The graph represents the quantification of positive staining as percentage compared to CTR () and is representative for 3 independent donors. Data are presented as mean ± SEM. Student’s 𝑡-test was used for single comparisons. (b) Mineral matrix deposition assayed by ARS (red staining) in siNURR1 and CTR cells after 21 days in osteogenic conditions. The graph shows the OD quantification of extracted dye from stained cell layers as percentage compared to CTR () and is representative for 3 independent donors. Data are presented as mean ± SEM. Student’s 𝑡-test was used for single comparisons.

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