Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2⁺/ABCB5⁺ Limbal Epithelial Stem Cell Cultures

Figure 3

Differentiation of ABCG2⁺/ABCB5⁺ LESCs into corneal epithelial cells. (a) ABCG2⁺/ABCB5⁺ LESCs were cultured in 10% DMEM or CnT30 and stained with cell-specific markers. (b) ABCG2⁺/ABCB5⁺ LESCs were cultured on transwell filters in 10% DMEM or CnT30 and stained with cell-specific markers. Scale bars = 100 μm and 200 μm.

(a)

(b)