Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2⁺/ABCB5⁺ Limbal Epithelial Stem Cell Cultures

Figure 4

Differentiation of ABCG2⁺/ABCB5⁺ LESCs into corneal epithelial cells with a transwell system. (a) Sections of ABCG2⁺/ABCB5⁺ LESCs cultured on transwell filters were stained with hematoxylin and eosin. (b) ABCG2⁺/ABCB5⁺ LESCs were cultured onto transwell filters in 10% DMEM, and transwell sections were stained with cell-specific markers. Scale bar = 100 μm. w/o Ab: without primary antibody + secondary antibody.

(a)

(b)