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Stem Cells International
Volume 2017 (2017), Article ID 7678637, 12 pages
https://doi.org/10.1155/2017/7678637
Research Article

Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2+/ABCB5+ Limbal Epithelial Stem Cell Cultures

1Department of Ophthalmology, Corneal Dystrophy Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
2Institute of Vision Research, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Republic of Korea
3Department of Obstetrics and Gynecology, Institute of Women’s Life Medical Science, Yonsei University College of Medicine, Seoul, Republic of Korea

Correspondence should be addressed to Yong-Sun Maeng; moc.liamg@wehttamgneam

Received 26 June 2017; Revised 27 September 2017; Accepted 12 October 2017; Published 5 November 2017

Academic Editor: Ming Li

Copyright © 2017 Eung Kweon Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: Cultivation of LECs in various culture conditions. Isolated LECs cultured on 5% matrigel, 0.05 mg/ml fibronectin, or a mixture of 5% matrigel + 0.05 mg/ml fibronectin in CnT20 or 10% DMEM for 15 days. Scale bar = 100 μm. Figure S2: Stem cell marker analysis of ABCG2+/ABCB5+ LESCs. Total mRNA was isolated from ABCG2+/ABCB5+ LESCs cultured in 10% DMEM, CnT20, or CnT30 and gene expression was assessed by RT-PCR. ∗∗ p < 0.01 vs DMEM. Figure S3: Colony formation analysis of ABCG2+/ABCB5+ LESCs. ABCG2+/ABCB5+ LESCs were seeded and cultured for 8 days. Colony formation was monitored by staining with antibodies to ABCG2 and P63α. Scale bar = 500 μm and 200 μm. Supplementary Table S1.

  1. Supplementary material