WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells

Figure 2

Efficient knockdown of WDR62 expression in hPSC-derived neural progenitors. (a) Map of inducible lentiviral shRNA vector and targeted sequence of WDR62. (b) GFP and corresponding brightfield images of WDR62 shRNA-transduced hPSC-derived neural progenitors with versus without Dox treatment at neural induction and NSP stages. (c) Gene expression analyses showing downregulation of WDR62 expression in WDR62 shRNA-transduced hPSC-derived neural progenitors treated with Dox versus no Dox treatment at neural induction () and NSP stages () ( and independent experiments, resp., >3 NSPs per experiment). Two-tailed t-test was used to assess significance. (d) Diameter measurements of WDR62 shRNA-transduced hPSC-derived NSPs treated with Dox versus no Dox controls ( independent experiments, >3 NSPs per experiment). Mann–Whitney U test was used to assess significance. ; . NI: neural induction; NSPs: neurospheres; Dox: doxycycline.

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