WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells

Figure 4

WDR62 knockdown effects differentiation to astrocytes. (a) Schematic diagram outlining protocol for hPSC differentiation to astrocytes. (b) GFP, brightfield, and S100β immunostaining images of WDR62 shRNA-transduced hPSC-derived astrocytes with versus without Dox treatment at the end of differentiation. DAPI stains of nuclei are shown. Scale bars = 50 μm. (c) Quantification analyses of S100β-positive cells suggesting significantly decreased () differentiation to astrocytes in Dox-treated group versus no Dox treatment controls ( independent experiments). Two-tailed t-test was used to assess significance. . Dox: doxycycline.

(a)

(b)

(c)