Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status

Figure 1

Time schedules of hiPS induction using an all-in-one retroviral vector (a) or four Sendai viral vectors (b). Fibroblasts from a manifesting female carrier of DMD (609) were infected with a retroviral vector, encoding five reprogramming factors. After replating onto MMC-treated STO feeder cells, colonies were picked up (a). Next, fibroblasts of 609 and a female DMD patient (386) were infected with four Sendai viral vectors on SNL feeders, and ES-like colonies were picked up 14–21 days after transduction (b).

(a)

(b)