Stem Cells International / 2017 / Article / Fig 4

Research Article

Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status

Figure 4

Loss of XIST signals in 609-iPS cells (XX) and 386-iPSCs (XXX). (a) Representative images of XIST RNA FISH (red)/X chromosome-DNA FISH (green) on 609 fibroblasts, 609-2 iPS cells (passage 5: p5), and 609S-1 iPS cells (p10). 609 fibroblasts have one XIST signal. Most 609-2 iPS cells retain XIST expression. In contrast, 609S-1 iPS cells, which were generated with Sendai viral vectors, had totally lost XIST expression. Quantitative data are also shown in Table 2. (b) Representative images of XIST RNA FISH (red)/X chromosome-DNA FISH (green) on 386 fibroblasts, and 386-S19 iPS cells. 386 fibroblasts have three X and two XIST signals. 386-S19 iPS cells, which were induced using Sendai viral vectors retained one XIST expression. Trimethyl-histone H3 (Lys27) (H3K27me3) signals (red) in 386-S19 iPS cells are shown. Most cells have just one H3K27me3 signal/cell. (c) XIST signals per cell in 386 fibroblasts and four 386 iPS cells. 386 fibroblasts have three X chromosomes and two XIST signals. In four 386 iPS clones, which were induced with Sendai viral vectors, most cells have one XIST signals.
(a)
(b)
(c)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.