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Stem Cells International
Volume 2017, Article ID 8085462, 10 pages
https://doi.org/10.1155/2017/8085462
Research Article

Angiogenic Capacity of Dental Pulp Stem Cell Regulated by SDF-1α-CXCR4 Axis

1Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea
2Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul 06351, Republic of Korea
3Single Cell Network Research Center, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea
4Laboratory of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea
5Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Republic of Korea
6Department of Anatomy & Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea
7Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea

Correspondence should be addressed to Kyunghoon Lee; ude.ukks@hkeel and Sun-Ho Lee; moc.liamg@27attobos

Received 16 December 2016; Revised 26 February 2017; Accepted 1 March 2017; Published 15 May 2017

Academic Editor: Dominique Bonnet

Copyright © 2017 Hyun Nam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. The expression pattern of perivascular markers in DPSCs. After completion of semi-quantitative PCR, PCR products for each gene were separated and visualized. Supplementary Figure 2. The effect of DPSC-CM on the migration of HUVECs. The migration of HUVECs by DPSC-CM was determined by wound healing assay. After culturing of HUVECs with various concentrations of DPSC-CM (0%, 25%, 50%, and 100%) for 9 hours, the migration ability of HUVECs was analyzed. (A) The migration of HUVECs was increased in the dose-dependent manner of DPSC-CM.(B) When 10 μM AMD3100 was treated with DPSC-CM, we could observe decreased migration ability of HUVECs.(C) Relative wound closure was calculated. *P<0.05, significantly different from 10 μM AMD3100 treatment. Supplementary Figure 3. The effect of DPSC-CM on the survival of HUVECs. The survival of HUVECs by DPSC-CM was determined by apoptosis assay.(A) After culturing of HUVECs with various concentrations of DPSC-CM (0%, 25%, 50%, and 100%), the survival ability of HUVECs was analyzed. DPSC-CM could increase survival of HUVECs in dose-dependent manner. Y-axis was arbitrary unit which was normalized with the value at day 0. #P<0.05, significantly different from 0% DPSC-CM; *P<0.05, significantly different from 100% DPSC-CM(B) The effect of each concentration of DPSC-CM on the survival of HUVECs was shown separately. *P<0.05, significantly different from 10 μM AMD3100 treatment.Supplementary Table 1. Antibodies for FACS analysis. Supplementary Table 2. Primers for qPCR.

  1. Supplementary Material