In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging

Figure 4

Chemotaxis assay for MSC-CXCR4/Fluc2. MSCs and MSC-CXCR4/Fluc2 in serum-free medium were placed into the upper well of a 24-well Transwell plate, and 0.5% FBS was added to the lower well. The plates were incubated for 6 h, and then cells that migrated into the lower well were fixed, stained with crystal violet, and photographed in 4x magnification. One representative experiment of three is shown.