Research Article

Therapeutic Potential of Multilineage-Differentiating Stress-Enduring Cells for Osteochondral Repair in a Rat Model

Table 3

Sellers scale for histological assessment of the repaired tissue.

Filling of the defect relative to surface of normal adjacent cartilage
111%–125%1
91%–110%0
76%–90%1
51%–75%2
26%–50%3
<25%4
Integration of repair tissue with surrounding articular cartilage
Normal continuity and integration0
Decreased cellularity1
Gap or lack of continuity on one side2
Gap or lack of continuity on two sides3
Matrix staining with safranin O/fast green
Normal0
Slightly reduced1
Moderately reduced2
Substantially reduced3
None4
Cellular morphology (choose first between a, b, c, and d)
(a) Normal0
(b) Mostly round cells with the morphology of chondrocytes
>75% of tissue with columns in radial zone0
25%–75% of tissue with columns in radial zone1
<25% of tissue with columns in radial zone (disorganized)2
(c) 50% round cells with the morphology of chondrocytes
>75% of tissue with columns in radial zone2
25%–75% of tissue with columns in radial zone3
<25% of tissue with columns in radial zone (disorganized)4
(d) Mostly spindle-shaped (fibroblast-like) cells5
Architecture within entire defect (not including margins)
Normal0
1–3 small voids1
1–3 large voids2
>3 large voids3
Clefts or fibrillations4
Architecture of surface
Normal0
Slight fibrillation or irregularity1
Moderate fibrillation or irregularity2
Severe fibrillation or disruption3
Percentage of new subchondral bone
90%–100%0
75%–89%1
50%–74%2
25%–49%3
<25%4
Formation of tidemark
Complete0
75%–99%1
50%–74%2
25%–49%3
<25%4