In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study

Figure 3

Human dental pulp stem cells (hDPSCs) were isolated and cultured in proliferation (a–d) or chondrocyte differentiation culture medium (e–h) for up to 6 weeks. F-Actin expression (a, e) was analyzed by fluorescence microscopy using rhodamine-conjugated phalloidin, and COLII (b, f), ACAN (c, g), and COLI (d, h) expression were analyzed by immunofluorescence. In all panels, cell nuclei were stained with DAPI. Microphotographs are representative fields of three independent experiments.

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