Capacity of Human Dental Follicle Cells to Differentiate into Neural Cells In Vitro

Figure 4

Neurosphere generation of hDFCs. (a) A floating cell sphere of hDFCs cultured in serum-free medium with FGF, EGF, and B27 supplement for 3 days. (b) The cells spread out from the neurosphere that adhered to the fibronectin-coated dish on day 0 of culture in NDM. (c) The cells extended from the neurosphere on day 3 of culture in NDM. (d) The cells extended from the neurosphere on day 7 of culture in NDM. Scale bars (a, b) = 100 μm and (c, d) = 50 μm. Arrows indicate neural-like cells with branched dendrites and refractile cell bodies.

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