Research Article
Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling
Figure 3
EtOH upregulates mTOR phosphorylation in DPCs in a dose-dependent manner. (a) DPCs were treated with different concentrations of EtOH for 24 hours. Lower panels show the ratios of band densities of phosphor-mTOR to mTOR. (b) Confluent DPCs cells were preincubated with the mTOR inhibitor rapamycin (50 mM) for 1 hour before treatment with EtOH. Rapamycin decreases EtOH-induced mTOR phosphorylation. Data represent mean ± SD of 3 experiments with triplicates. versus control group. versus EtOH group.
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