Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2017, Article ID 8920356, 14 pages
Research Article

Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

1Department of Endodontics, Faculty of Medicine and Dentistry, Catholic University of Valencia “San Vicente Mártir”, C/Quevedo, 2, 46001 Valencia, Spain
2Center for Biomedical Network Research on Rare Diseases (CIBERER), CIBER-ISCIII, Valencia, Spain
3Department of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Av. Blasco Ibañez 15, 46010 Valencia, Spain
4INCLIVA Health Research Institute, Av. de Menéndez y Pelayo 4, 46010 Valencia, Spain
5Cell Culture Laboratory, Central Unit of Research in Medicine (UCIM), University of Valencia, Av. Blasco Ibañez 15, 46010 Valencia, Spain

Correspondence should be addressed to Alejandro Victoria-Escandell; se.vcu@airotciv.ordnajela

Received 17 January 2017; Revised 22 March 2017; Accepted 12 April 2017; Published 24 May 2017

Academic Editor: Andrea Ballini

Copyright © 2017 Alejandro Victoria-Escandell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex). Cell viability and cell death rate were assessed by flow cytometry. Oxidative stress was measured by OxyBlot. Levels of antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by quantifying the expression levels of DNA damage sensors such as ATM and RAD53 genes and DNA damage repair sensors such as RAD51 and PARP-1. Results indicate that AH-Plus increased apoptosis, oxidative stress, and genotoxicity markers in HDPSCs. MTA-Fillapex was the most cytotoxic oxidative stress inductor and genotoxic material for HDPSCs at longer times in preincubated cell culture medium, and MTA-Angelus was less cytotoxic and genotoxic than AH-Plus and MTA-Fillapex at all times assayed.