Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials

Figure 2

Oxidative stress and antioxidant responses in HDPSCs incubated with endodontic materials. Immunoblots are representative images of two independent analyses in HDPSCs incubated with MTA-Angelus, AH-Plus, and MTA-Fillapex. (a) OxyBlot analysis for the detection of carbonylated proteins from total extracts of HDPSCs incubated with endodontic materials for 24 h. (b) OxyBlot analysis for the detection of carbonylated proteins from total extracts of HDPSCs incubated with endodontic materials for 7 days. (c) Western blot analysis of two antioxidant enzymes, catalase and MnSOD, from total extracts of HDPSCs incubated with endodontic materials for 24 h. For OxyBlots, Coomassie gel staining was used as a loading control. In immunoassays for detecting the levels of antioxidant enzymes, β-actin was used as a reference and loading control.

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