Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9

Figure 1

Effect of single guide sequence for hMYOGENIN by the CRISPR/Cas9 system. A schematic representation of MYOG exons and introns. A candidate position for Cas9 targeting of MYOG exon1 (a). pX458-hMYOG+189, a construct for driving single guide RNA bound to MYOG exon1 and bicistronic expression of both Cas9 and GFP (b). T7 endonuclease I assay for Cas9-mediated cleavage (arrows, 500 bp and 300 bp) on an agarose gel, showing comparable modification of the targeted human MYOG genomic fragment in HEK293T cells (c). Relative expression of MYOG in Hu5-immortalized human myoblast cells transfected with or without the pX458-hMYOG+189 vector. All error bars indicate ±SEM ().

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