Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9

Figure 4

Myogenic differentiation from mesodermal precursors derived from MYOGENIN-deficient hiPS clone number 28 cells. Mesodermal and myogenic differentiation flowchart of the time course for muscle cells derived from hiPS cells (a). FACS analyses of DLL1-positive mesodermal cells derived from hiPS cells (b). Relative expression of total MYOD1 and endogenous MYOD1 (c). Differentiated myogenic cells derived from mesodermal cells with or without MYOG for 60 days were immunostained with anti-MYOSIN HEAVY CHAIN (MYHC, green) antibody. Nuclei were stained with 4′6-diamidino-2-phenylindole (DAPI, blue). Scale bar, 100 μm (d). Relative expressions of MYOD1 and MRF4 transcripts in wild-type or MYOG-mutated myogenic cells derived from mesodermal cells (e). All error bars indicate ±SEM (). values are determined by a t-test from a two-tailed distribution. , .

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