Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9

Table 2

Primer sequences for T7 endonuclease assay and quantitative RT-PCR.

Genes	Sequences	Amplicon size
Primer for T7 EI assay
MYOG gDNA_F	5′-GGCCGCCCAGCTAGGAGTAATTGA-3′	786
MYOG exon1_R	5′-CGCTCGATGTACTGGATGGCACTG-3′
Primer for RT-qPCR
RPL13A_F	5′-CCCTGGAGGAGAAGAGGAAA-3′	91
RPL13A_R	5′-ACGTTCTTCTCGGCCTGTTT-3′
MYOG_F	5′-GCTCAGCTCCCTCAACCA-3′	94
MYOG_R	5′-GCTGTGAGAGCTGCATTCG-3′
MYOD1_F	5′-GCACTACAGCGGCGACTCC-3′	118
MYOD1_R	5′-GTAGGCGCCTTCGTAGCAG-3′
Endo-MYOD1_F	5′-CACTCCGGTCCCAAATGTAG-3′	180
Endo-MYOD1_R	5′-TTCCCTGTAGCACCACACAC-3′
MRF4_F	5′-GGCCAAGTGTTTCCGATCAT-3′	89
MRF4_R	5′-AAGGCTACTCGAGGCTGACG-3′