Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid

Figure 2

Cell proliferation and HF marker expression in aggregates: (a) mixed DP-KC aggregates; (b) coated DP-KC aggregates; and (c) LF-KC mixed aggregates as a control. Confocal microscopy. DP cells and LF are labelled with RFP. Immunocytochemistry for Ki67, P cadherin, Lef1, and EpCAM as indicated (green). White arrows point to Lef1 and Ki67 positive nuclei; white dotted lines show the borderline between DP cells and KCs. Nuclei are counterstained with TOTO3 or DRAQ5 (cyan color). Scale bar 50 μm for Ki67 and Lef1 staining and 100 μm for P cadherin and EpCAM staining. Dashed lines show the borderline between initial DP aggregate and KCs.

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