Effect of Isolation Technique and Location on the Phenotype of Human Corneal Stroma-Derived Cells

Figure 1

Anatomical features of the human cornea and sites of stromal cell isolation. Pieces of tissue were punched out from the indicated central and peripheral corneal regions by a surgical trephine (3 mm) for consequent digestion and/or culturing. The picture was taken by a phase contrast microscope and put together as an overlay (EVOS® FL microscope, Thermo Fisher Scientific).