Effect of Isolation Technique and Location on the Phenotype of Human Corneal Stroma-Derived Cells

Figure 6

Gene expression profile of the native corneal stroma compared to cultured CSCs. Relative quantities are shown on a logarithmic scale, in the native and pooled cultured cells from the different isolation/cultivation methods; mean relative quantity (RQ) ± standard deviation (SD) is represented. Significance values are depicted as and . 18S RNA was used as a housekeeping gene, with the expression levels of the cultured CSCs compared to that of the native tissue (). The RNA from the native tissue was prepared by mincing the stromal tissue from 3 donors into pieces, followed by extraction of total RNA.