Research Article

Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study

Figure 3

Detection of cell surface markers expressed by SVF cells and ASC at passages 0 and 1 by immunophenotyping and flow-cytometric analysis. (a) Gating strategy stream on morphological forward (FSC) and side scatter (SSC) used to eliminate debris and selected for cultured hASCs represented by contour plot. (b) Immunophenotyping of SVF cells. (c) Immunophenotyping of ASC at the first subculture (P0). (d) Immunophenotyping of ASC at the second subculture (P1). ; .
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