Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study

Figure 4

Growth kinetics and clonogenic potential of cells obtained with the four methods. (a) Population doubling (PD) at passages 1 and 2 and the corresponding cumulative PD for the four methods used to obtain the SVF. (b) Doubling time in hours for each subculture. (c) Representative photograph of a clone for CFU assay. (d) Colony forming efficiency calculated based on CFU assay. (e) All cell lines showed a normal karyotype with 23 chromosomes pairs and G-banding, indicating no chromosomal aberrations. (The karyotype shown is representative of the four methods evaluated.) (f) No hTERT expression was detected in the cell lines tested while positive control MRC5p hTERT-transfected cell line showed a high expression. CFE, colony forming efficiency; ND, not detected; NS, not significant. .

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