Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study

Figure 5

Multilineage differentiation of passage 1 adipose-derived stromal cell isolated using four different methods. (a) Cells either untreated (Ctl) or treated with adipogenic induction medium (IM) for 14 days and stained with Oil Red O. (b) RT-PCR assay for leptin and PPARG adipogenic specific genes. (c) Cells either untreated (Ctl) or treated with osteogenic induction medium (IM) for 21 days and stained with Alizarin Red. (d) RT-PCR assay for ALPL and RUNX2 osteogenic specific genes. (e) Cells either untreated (Ctl) or treated with chondrogenic induction medium (IM) for 21 days and stained with Alcian Blue. Scale bar 200 μm. (f) RT-PCR assay for COL2A1, ACAN, and SOX9 chondrogenic specific genes.

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