Evaluation of Three Devices for the Isolation of the Stromal Vascular Fraction from Adipose Tissue and for ASC Culture: A Comparative Study

Table 1

List of devices for SVF isolation.

Device	Company	Open/; semiclosed/; closed	Automated/; semiautomated/; manual	Maximum fat quantity (g)	Method for fluid separation	Collagenase provided (yes/no)	Disposable cost ($)	Fat treatment duration (minutes)	Publication
Celution® 800/CRS	Cytori Therapeutics, Inc.; http://www.cytori.com	Semiclosed	Automated	300	Decantation	Yes; (Celase™)	1950	90	[8]
Cha-Station™	PNC International Co., Ltd.; http://inphronics.com.sg/equipment/cha-station	Semiclosed	Semiautomated	200	Not provided	No	710	90	[8]
GID SVF-1	GID Group, Inc.; http://www.thegidgroup.com/	Closed	Manual	300	Filtration	Yes; (GIDzyme-2)	500	90	[24, 28]
Lipokit with Maxstem	Medi-Khan; http://www.medikanint.com/	Semiclosed	Manual	100	Centrifugation	No	530	110	[8]
Multi Station	PNC International Co., Ltd.; PNC North America; http://pncint.business1.com/	open	Manual	100–150	Centrifugation	No	460	110	[8]
Puregraft 250	Eurosilicone	Semiclosed	Manual	250	Filtration	No	250	100^†	[23]
Sepax®	Biosafe Group SA; https://www.biosafe.ch	Semiclosed	Semiautomated	300	Not provided	No	420	90–120	[29]
Stem.pras with Duografter II	Proteal	closed	Manual	200^†	Decantation	No	Unknown	110^†	None
Tissue Genesis® Icellator	Tissue Genesis, Inc.; http://www.tissuegenesis.com/icellator	Semiclosed	Semiautomated	60	Not provided	Yes; Adipase®	Unknown	80	[9]

added from this study
relies to those that do not allow the lipoaspirate to be harvested direct in the device.