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Stem Cells International
Volume 2017 (2017), Article ID 9405204, 11 pages
Research Article

Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

1Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, China
2Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, China
3School of Medicine, Jiaxing University, Zhejiang 314001, China
4Changzheng Hospital, Second Affiliated Hospital of Second Military Medical University, Shanghai 200003, China
5Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Tongji University Advanced Institute of Translational Medicine, Shanghai 200092, China

Correspondence should be addressed to Xinxin Han; nc.ude.naduf@nahxx, Hua He; moc.anis@hh9791adnap, and Zhengliang Gao; nc.ude.ijgnot@oag_gnailgnehz

Received 19 May 2017; Revised 28 June 2017; Accepted 27 July 2017; Published 18 September 2017

Academic Editor: Yao Li

Copyright © 2017 Xinxin Han et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.