Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

Figure 1

Flow chart of method used to obtain neural stem cell-like cells derived from human embryonic stem cells. (a) Differentiation process of human embryonic stem cells to neural stem cells. (b) State of cells in different differentiation stages. Human embryonic stem cells originally grew on feeder cells, and then cells were digested to single cell and removed the feeder cells. Single hES cells stuck to the dish bottom surface and grew to single-layer hES cells. Reduced medium and the factors were added to the single-layer hES cells. The cells were cultured until the end of differentiation. The process lasted about 4 weeks, and the cells were kept carefully in a fresh medim. KSR media: knockout serum replacement media; MEF-C media: mouse embryonic fibroblast cell conditional medium; non-ad hES: nonadherent human embryonic stem cells.