Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro

Figure 2

Secretion of matrix metalloproteinase- (MMP-) 1 (a), MMP-3 (b) on day 7, MMP-13 (c, d) on days 3 and 7, MMP-2 (e), and MMP-9 (f) on day 7 (in ng/mL), determined by ELISA in supernatants of cartilage explants (CE), ASC, and in cocultures (CE + ASC). IM—incomplete chondrocyte medium, IM + PP—IM supplemented with human platelet growth factor-enriched plasma (PP), and IL-1β—interleukin-1β. Data presented as mean ± standard deviation; ; ; ND—not detected. The levels of MMPs obtained from day 3 experiments in the most of cases were similar to day 7; therefore, only day 7 demonstrated.

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