Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro
Table 2
The levels of secreted factors related to inflammation and chondrogenesis.
Secreted factor, coculture duration, and medium conditions
Concentration, ng/mL
ASC
CE
ASC + CE
VCAM-1
3 d. IM + PP
0.0
20.9 ± 8.4
25.7 ± 13.8
3 d. IM + PP + IL-1β
2.1 ± 1.5
83.3 ± 49.8
32.6 ± 13.4
7 d. IM + PP
7.3 ± 12.7
43.5 ± 27.6
39.6 ± 28.7
7 d. IM + PP + IL-1β
4.2 ± 4.9
19.1 ± 7.1
13.8 ± 11.6
TSP-1
7 d. IM + PP
2519.1 ± 1164.4
2031.1 ± 1599.5
1729.5 ± 769.6
7 d. IM + PP + IL-1β
2076.0 ± 1565.0
1226.1 ± 239.0
1697.4 ± 783.4
MIA
3 d. IM + PP
0.1 ± 0.0
14.4 ± 13.1
28.6 ± 19.5
3 d. IM + PP + IL-1β
0.1 ± 0.2
6.2 ± 6.9
18.4 ± 14.1
7 d. IM + PP
0.0 ± 0.0
35.6 ± 12.6
24.0 ± 18.44
7 d. IM + PP + IL-1β
0.0 ± 0.0
16.9 ± 9.3
14.4 ± 13.4
Aggrecan
3 d. IM + PP
0.0
4.0105 ± 2.3105
4.1105 ± 1.4105
3 d. IM + PP + IL-1β
0.0
3.8105 ± 1.1105
4.4105 ± 2.2105
7 d. IM + PP
0.0
5.0105 ± 1.6105
5.6105 ± 1.9105
7 d. IM + PP + IL-1β
0.0
3.5105 ± 6.9105
4.5105 ± 1.8105
Glycosaminoglycan
7 d. IM + PP
1.1 ± 0.5
93.9 ± 26.1
96.4 ± 38.1
7 d. IM + PP + IL-1β
1.5 ± 0.7
114.7 ± 22.3
97.8 ± 24.1
TGF β1
3 d. IM + PP
2120.2 ± 1106.9
17753.3 ± 2060.0
15901.3 ± 7041.7
3 d. IM + PP + IL-1β
2995.9 ± 527.8
14087.7 ± 944.2
17175.4 ± 2281.5
7 d. IM + PP
2965.7 ± 481.4
19203.6 ± 933.7
18099.8 ± 5582.5
7 d. IM + PP + IL-1β
2694.8 ± 816.8
17122.7 ± 2230.1
14212.5 ± 6798.3
Table 2 represents the levels of vascular cell adhesion molecule 1 (VCAM-1), thrombospondin-1 (TSP-1), melanoma inhibitory activity (MIA), aggrecan, glycosaminoglycan, and transforming growth factor (TGF) β1, measured by ELISA in supernatants of cartilage explants (CE), adipose tissue-derived stem cells (ASC), and cocultures (CE + ASC). Incomplete chondrogenic medium supplemented with human platelet growth factor-enriched plasma—IM + PP, and supplemented with IL-1β—IM + PP + IL-1β. , when compared to ASC + CE versus CE.
