Figure 1: Increased expression of anti-inflammatory genes in preactivated mesenchymal stem cells. Gene expression analysis of murine MSCs was performed after preactivation with 20 ng/mL IFN-γ and 30 ng/mL TNF-α for 20 h. Expression changes on Il-6, Pd-l1 (CD274), iNos, Ido, Cox-2, and Mmp2 were analyzed by qPCR on preactivated cells and compared to the control group (untreated cells). Data were expressed as fold change ± SD of three independent experiments.. One-way ANOVA, post hoc Tukey.