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Stem Cells International
Volume 2017, Article ID 9824698, 12 pages
https://doi.org/10.1155/2017/9824698
Research Article

In Vivo Immunogenic Response to Allogeneic Mesenchymal Stem Cells and the Role of Preactivated Mesenchymal Stem Cells Cotransplanted with Allogeneic Islets

1Stem Cell Laboratory, Fundamental Health Science Institute, Universidade Federal Rio Grande do Sul, Porto Alegre, RS, Brazil
2Post Graduate Program in Physiology, Universidade Federal Rio Grande do Sul, Porto Alegre, RS, Brazil
3Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brazil
4Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal Rio Grande do Sul, Porto Alegre, RS, Brazil
5Stem Cell Research Institute, Porto Alegre, RS, Brazil

Correspondence should be addressed to Patricia Pranke; rb.sgrfu@eknarpaicirtap

Received 26 October 2016; Revised 6 February 2017; Accepted 8 February 2017; Published 3 May 2017

Academic Editor: Benedetta Bussolati

Copyright © 2017 Régis Linhares Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table S1. Primer sequences and amplicon characteristics. Figure S1. Characterization of adipose-derived mesenchymal stem cells at passage 5. (A) The cells were immunophenotyped for the expression of CD11b, CD31, CD44, CD45, CD90.2 and Sca-1 by flow cytometry. At least 5.000 events were analyzed. (B) Cell morphology was analyzed by phase contrast (B) and fluorescence microscopy (C). Adipogenic and osteogenic differentiation of adipose-derived mesenchymal stem cells at passage 5. MSCs cultured for 4 weeks in adipogenic (D) and osteogenic (F) media and respective control group (E, G). Cells stained with Oil Red O (D, E) and (F, G) Alizarin Red S. Magnification 100X.

  1. Supplementary Material