Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

Figure 1

MSC characterization. (a) Cytometry graphs in solid black curves refer to the negative controls (cells incubated with secondary antibody) and nonfilled curves indicate the marker tested. The cell population expressed CD90, 73, and 105 and did not express CD19 and 45. (b) Brightfield images are showing that MSCs are able to differentiate in adipocytes, osteocytes, and chondrocytes. Undifferentiated cells were grown only in DMEM (A, C, and E) and show only background staining for oil red O on (A), Von Kossa on (C), and alcian blue on (E). Once incubated in differentiation-inducing culture mediums, adipose-derived MSCs showed positive staining for oil red O on (B), Von Kossa on (D), and for alcian blue on (F). Scale Bar = 200 μm. Representative images of three biological replicates that were conducted independently.

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